Question 135: Wave Optics

Question

The value of numerical aperature of the objective lens of a microscope is 1.25. If light of wavelength 5000 $\mathop A\limits^o $
is used, the minimum separation between two points, to be seen as distinct, will be :

Accepted Answer

Numerical aperature of the microscope is given as
$NA = {{0.61\lambda } \over d}$
Where d = minimum sparaton between two points to be seen as distinct
$d = {{0.61\lambda } \over {NA}} = {{\left( {0.61} ight) 	imes \left( {5000 	imes 10\,{m^{ - 10}}} ight)} \over {1.25}}$
= 2.4 $ 	imes {10^{ - 7}}\,m = 0.24\,\mu m$

Answer

0.12 $\mu $m

Answer

0.38 $\mu $m

Answer

0.24 $\mu $m

Answer

0.48 $\mu $m

Wave Optics

The value of numerical aperature of the objective lens of a microscope is 1.25. If light of wavelength 5000 \mathop A\limits^o is used, the minimum separation between two points, to be seen as distinct, will be :

Choose the correct answer: